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cre dependent flp recombinase  (Addgene inc)


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    Structured Review

    Addgene inc cre dependent flp recombinase
    Cre Dependent Flp Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+dependent+flp+recombinase/pAAV-TRE-DIO-FLPo+(Plasmid+%23118027)/pm41505254-293-75-81
    Average 94 stars, based on 10 article reviews
    cre dependent flp recombinase - by Bioz Stars, 2026-09
    94/100 stars

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    Software:

    Article Title: Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro 6 WaveMetrics version 6.1.2.1 MATLAB MathWorks R2019a Excel Microsoft 365 2021 ImageJ Schneider et al. 129 https://imagej.nih.gov/ij/NIH SNT Arshadi et al. 130 https://imagej.net/plugins/snt Python 3.9.0 Python Software Foundation https://www.python.org Original code for analysis N/A https://doi.org/10.5281/zenodo.17683276 20 Cell Reports 45, 116792, January 27, 2026 Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system 123 : pTREDIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely (https://www.addgene.org/118027/); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system, 124 was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling 125 (https://www.addgene.org/161577/), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA.

    Construct:

    Article Title: Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro 6 WaveMetrics version 6.1.2.1 MATLAB MathWorks R2019a Excel Microsoft 365 2021 ImageJ Schneider et al. 129 https://imagej.nih.gov/ij/NIH SNT Arshadi et al. 130 https://imagej.net/plugins/snt Python 3.9.0 Python Software Foundation https://www.python.org Original code for analysis N/A https://doi.org/10.5281/zenodo.17683276 20 Cell Reports 45, 116792, January 27, 2026 Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system 123 : pTREDIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely (https://www.addgene.org/118027/); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system, 124 was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling 125 (https://www.addgene.org/161577/), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA.

    Article Title: Cell-type-selective synaptogenesis during the development of excitatory connectivity in the mammalian neocortex
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system : pTRE-DIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely ( https://www.addgene.org/118027/ ); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system , was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling ( https://www.addgene.org/161577/ ), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA .

    Labeling:

    Article Title: Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro 6 WaveMetrics version 6.1.2.1 MATLAB MathWorks R2019a Excel Microsoft 365 2021 ImageJ Schneider et al. 129 https://imagej.nih.gov/ij/NIH SNT Arshadi et al. 130 https://imagej.net/plugins/snt Python 3.9.0 Python Software Foundation https://www.python.org Original code for analysis N/A https://doi.org/10.5281/zenodo.17683276 20 Cell Reports 45, 116792, January 27, 2026 Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system 123 : pTREDIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely (https://www.addgene.org/118027/); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system, 124 was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling 125 (https://www.addgene.org/161577/), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA.

    Article Title: Cell-type-selective synaptogenesis during the development of excitatory connectivity in the mammalian neocortex
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system : pTRE-DIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely ( https://www.addgene.org/118027/ ); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system , was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling ( https://www.addgene.org/161577/ ), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA .

    Modification:

    Article Title: Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro 6 WaveMetrics version 6.1.2.1 MATLAB MathWorks R2019a Excel Microsoft 365 2021 ImageJ Schneider et al. 129 https://imagej.nih.gov/ij/NIH SNT Arshadi et al. 130 https://imagej.net/plugins/snt Python 3.9.0 Python Software Foundation https://www.python.org Original code for analysis N/A https://doi.org/10.5281/zenodo.17683276 20 Cell Reports 45, 116792, January 27, 2026 Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system 123 : pTREDIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely (https://www.addgene.org/118027/); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system, 124 was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling 125 (https://www.addgene.org/161577/), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA.

    Article Title: Cell-type-selective synaptogenesis during the development of excitatory connectivity in the mammalian neocortex
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system : pTRE-DIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely ( https://www.addgene.org/118027/ ); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system , was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling ( https://www.addgene.org/161577/ ), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA .

    Electroporation:

    Article Title: Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro 6 WaveMetrics version 6.1.2.1 MATLAB MathWorks R2019a Excel Microsoft 365 2021 ImageJ Schneider et al. 129 https://imagej.nih.gov/ij/NIH SNT Arshadi et al. 130 https://imagej.net/plugins/snt Python 3.9.0 Python Software Foundation https://www.python.org Original code for analysis N/A https://doi.org/10.5281/zenodo.17683276 20 Cell Reports 45, 116792, January 27, 2026 Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system 123 : pTREDIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely (https://www.addgene.org/118027/); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system, 124 was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling 125 (https://www.addgene.org/161577/), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA.

    Article Title: Cell-type-selective synaptogenesis during the development of excitatory connectivity in the mammalian neocortex
    Article Snippet: 0.75 μL of buprenorphine (1 mg/mL; ZooPharm LLC) was administered for post-operative pain control. .. Constructs used for sparse labeling of L6CThNs for axon reconstruction were modified from the Supernova system : pTRE-DIO-FlpO (15 ng/μL), a Cre-dependent Flp recombinase that expresses sparsely ( https://www.addgene.org/118027/ ); and pCAG-FSF-turboRFP-ires-tTA (0.75 μg/μL), a Flp-dependent RFP. pCAG-LSL-EGFP (1.5 μg/μL), a Cre-dependent construct independent of the Supernova system , was electroporated at higher concentrations to provide denser labeling of L6CThNs for layer identification and monitoring of electroporation efficiency. .. In experiments testing the effect of suppressed neuronal activity on L6CThN morphology, pHSYN-FLEx-FRT-Kir2.1-2A-GFP (1.5 ng/μL), a Flp-dependent expression construct for Kir2.1 with GFP labeling ( https://www.addgene.org/161577/ ), was electroporated with pTRE-DIO-FlpO and pCAG-FSF-turboRFP-ires-tTA .



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